Por Autor (acceso a CvUy)
Prof. Adj. Dra. Adriana Cassina
Prof. Agr. Dra. Silvia Chifflet
Publicaciones Recientes del Departamento de Bioquímica (2005-2010)
Quijano C, Cassina A, Castro L, Rodríguez M and Radi R. Peroxynitrite: a mediator of nitric-oxide dependent mitochondrial dysfunction in pathology. In Nitric Oxide, Cell Signaling, and Gene Expression (Cadenas E and Lamas S, Eds.)Marcel Dekker Inc, New York. Pp 99-143 (2005).
Romero N and Radi R. Hemoglobin and red blood cells as tools for studying peroxynitrite biochemistry. Methods in Enzymology 396, 229-245 (2005)
Denicola A and Radi R.Peroxynitrite and drug-dependent toxicity. Toxicology. 208:273-288 (2005)
Moller M, Botti H, Batthyany C, Rubbo H, Radi R and Denicola A. Direct measurement of nitric oxide and oxygen partitioning into liposomes and low density lipoprotein. J. Biol. Chem. 280: 8850-8854 (2005).
Batthyany C, Souza JM, Durán R, Cassina A, Cerveñansky C and Radi R. Time course and site(s) of cytochrome c tyrosine nitration by peroxynitrite. Biochemistry 44, 8038-8046,2005.
Quijano C, Romero N. and Radi R. Tyrosine nitration by superoxide and nitric oxide fluxes in biological systems: modeling the impact of superoxide dismutase and nitric oxide diffusion. Free Radical Biology and Medicine 39, 728-741,2005
Trujillo M, Folkes L, Bartesaghi S, Kalyanaraman B, Wardman P and Radi R. Peroxynitrite-derived carbonate and nitrogen dioxide radicals readily react with lipoic and dihydrolipoic acid. Free Radical Biology and Medicine. 39, 279-288, 2005.
López GV, Batthyány C, Blanco F.,Botti H, Trostchansky A, Migliaro E, Radi R, González M, Cerecetto H and Rubbo H. Design, synthesis and biological characterization of potential antiatherogenic nitric oxide releasing tocopherol analogs. Bioorganic & Medicinal Chemistry. 13:5787-5796, 2005.
Latini A, Rodríguez M, Borba Rosa R, Scussiato K, Leipnitz G, Reis De Assis D, Da Costa Ferreira G, Funchal C, Jacques-Silva MC, Buzzin L, Giugliani R, Cassina A, Radi R and Wajner M. 3-Hydroxyglutaric Acid Moderately Impairs Energy Metabolism In Brain of Young Rats. Neuroscience 135:111-120, 2005.
Naviliat M., Gualco G., Cayota A. and Radi R.Protein 3-nitrotyrosine formation during Trypanosoma cruzi infection in mice. Brazilian J Med Biol Res 38: 1825-1834, 2005
Nickel C, Trujillo M, Rahlfs S, Deponte M, Radi R, Becker K. Plasmodium falciparum 2-Cys peroxiredoxin reacts with plasmoredoxin and peroxynitrite Biol. Chem. 386 (11):1129-36, 2005
Rubbo, H. and O’Donnell, V.B Nitric oxide, peroxynitrite and lipoxygenase in LDL oxidation: Mechanistic insights.Toxicology 208, 305-317, 2005.
Botti, H., Trostchansky, A., Batthyany, C. and Rubbo, H. Reactivity of peroxynitrite and nitric oxide with LDL. IUBMB Life 57, 407-412, 2005.
Hurtado, J., Nin, N. and Rubbo, H. Stress oxidativo y nitrativo en la sepsis. Medicina Intensiva, España 29, 159-165, 2005.
Berriel, E., Hill, M., Barcia, J.J., Ubillos, L., González, M., Detjen, G., Rondán, M., Navarrete, H. and Osinaga, E. Simple mucin-type cancer associated antigens are pre-cancerous biomarkers during 1,2-dimethylhydrazine-induced rat colon carcinogenesis. Oncology Reports 14:219-228 (2005)
Medeiros, A., Plá, A., Alonso Paz, E., Batista-Viera, F., Osinaga, E. and Franco Fraguas, L. Identification of a high molecular weight glycoconjugate from Uruguayan plant which binds to the tumor-associated Tn antigen. Pharmaceutical Biology 43:26-32 (2005)
Varangot, M., Barrios, E., Sóñora, C., Aizen, B., Pressa, C., Estrugo, R., Laviña, R., Musé, I., Osinaga, E. and Berois, N. Clinical evaluation of a panel of mRNA markers in the detection of disseminated tumor cells in patients with operable breast cancer. Oncology Reports 14:537-545 (2005)
Freire, T, Bay S, von Mensdorff-Pouilly S, Osinaga, E. Molecular basis of incomplete O-glycan synthesis in breast cancer cells: putative role of MUC6 in Tn antigen expression. Cancer Research 65: 7880-7887 (2005)
Hill, M.; Pereira, V.; Chauveau, C.; Zagani, R.; Remy, S.; Tesson, L.; Mazal, D.; Ubillos, L.; Moffett, J.; Doebis, C.; Seifert, M.; Boczkowski, J.; Osinaga, E. and Anegon, I. Heme oxygenase-1 inhibits rat and human breast cancer cells proliferation: mutual cross-inhibition with indoleamine 2,3-dioxygenase FASEB Journal 19:1957-1968 (2005)
Chifflet S, Hernandez JA, Grasso S.
A possible role for membrane depolarization in epithelial wound healing. Am J Physiol Cell Physiol. 2005 288:C1420-30
Pineyro MD, Pizarro JC, Lema F, Pritsch O, Cayota A, Bentley GA, Robello C. Crystal structure of the tryparedoxin peroxidase from the human parasite Trypanosoma cruzi. J Struct Biol. 2005 Apr;150(1):11-22.
Oppezzo P, Dumas G, Lalanne AI, Payelle-Brogard B, Magnac C, Pritsch O, Dighiero G, Vuillier F. Different isoforms of BSAP regulate expression of AID in normal and chronic lymphocytic leukemia B cells. Blood. 2005 Mar 15;105(6):2495-503.
Grasso, S., Chifflet, S. and Hernández, J.A. 2007. Roles of the wound geometry, wound size and extracellular matrix in the healing response of bovine corneal endothelial cells in culture. Am. J. Physiol., 2007 Aug 8; [Epub ahead of print]
Torres, G., Charmantier-Daures, M., Chifflet, S., Anger, K. 2007. Effects of long-term exposure to different salinities on the location and activity of Na+-K+-ATPase in the gills of juvenile mitten crab, Eriocheir sinensis.Comp. Biochem. Physiol. A Mol. Integr. Physiol. 147:460-5
Ferrer-Sueta G, Hannibal L, Batinic-Haberle I and Radi R. Reduction of Manganese Porphyrins by Flavoenzymes and Submitochondrial Particles: A Catalytic Cycle for the Reduction of Peroxynitrite. Free Radical Biology and Medicine. 41, 503-512, 2006
Bartesaghi S, Valez V, Trujillo M, Peluffo G, Romero N, Zhang H, Kalyanaraman B and Radi R. Mechanistic studies of peroxynitrite-mediated tyrosine nitration in membranes using the hydrophobic probe N-t-BOC L-tyrosine tert butyl ester (BTBE). Biochemistry. 45: 6813-6825, 2006
Trujillo M, Mauri P, Benazzi L, Comini M, De Palma A, Flohé L, Radi R, Stehr M, Singh M, Ursini F and Jaeger T. The mycobacterial thioredoxin peroxidase can act as a one-cysteine-peroxiredoxin. J. Biol. Chem.281, 20555-20566, 2006
Radi R. Immuno-spin trapping: A breakthrough for the sensitive detection of protein-derived radicals [commentary]. Free Radical Biology and Medicine. 41, 416-417 , 2006
Radi R. How I Became a Biochemist. IUBMB Life, 58(9): 559 – 561, 2006
Romero N, Denicola A and Radi R. Red blood cells in the metabolism of nitric oxide-derived peroxynitrite. IUBMB-Life. 58: 572-580, 2006
Trostchansky; A, Lind S, Hodara, R., Ischiropouluos, H. Rubbo, H, and Souza, J.Interaction with phospholipids modulates α-synuclein nitration and lipid-protein adduct formation.Biochemical Journal, 393, 343-349, 2006
Trostchansky, A, O`Donnell, V.B., Goodwin, D, Landino, L, Marnett, L.J., Radi, R, Rubbo R Interactions between nitric oxide and peroxynitrite during prostaglandin endoperoxide H synthase – 1 catalysis: A free radical mechanism onf inactivation. Free Radicals Biology and Medicine, in press
Ferreira, A, Batthyany, C, Ferrari, M., Trostchansky , A, Souza. J, Alvarez M, López, V, O`Donell, V.B., Feeman, B. and Rubbo, H. Increased formation of cholesteryl-nitrolinoleate in activated macrophage: Down-regulation of iNOS and pro-inflammatory cytokines. Journal of Biological Chemistry, submitted -2006
Trostchansky, A., Souza, J., Ferreira, A., Ferrari, M., Blanco, F., Trujillo, M., Cerecetto, H., Baker, P., O`Donell, V.B., and Rubbo, H. Nitroarachidonate modulates inflammation: Characterization, isomer distribution and biological activity. Biochemistry, submitted- 2006
H. Rubbo, A. Trostchansky, J, Souza, A.Ferreira,H. Cerecetto, P. Baker and V. B. O`Donnell. Nitroarachidonate moulateinflammation: Isomes characterization: Isomer Characterization and biological activity. Free Rad. Biol.. Med. 41, S1, 2006 (Abstracts seleccionados publicados)- 2006
Hill,M. Pereira, V,Chauveau, C., Zagani, R., Remy, S., Tesson, L, Mazal D, Ubillos, L., Brion, R., Ashgar, K, Mashreghi, M., Kotsch, K., Moffett, J., Doebis, C., Seifert, M., Boczkowski, J., Osinaga, E, and Anegon, I. Heme oxigenase–1 inhibits rat and human breast cancer cells proliferation: mutual cross inhibition with indoleamine 2, 3-dioxygenase. FASEB J 2005 19:1957-68 –2006
Torre, M.H. Chifflet S. Foglia, M. 2006. Copper complexes with heterocyclic sulfonamides, potential drugs for dermatological diseases. /Metal Ions In Biology and Medicine, vol.:9. Paris: John Libbey Eurotext/, p. 59-62.- 2006
Bartesaghi S, Ferrer-Sueta G, Peluffo G, Valeria V, Zhang H, Kalyanaraman B and Radi R. Protein tyrosine nitration in hydrophilic and hydrophobic environments. Amino Acids 32: 501–515, 2007
Carballal S, Alvarez B, Turell L, Botti H, Freeman BA and Radi R. Sulfenic acid in human serum albumin. Amino Acids 32: 543–551, 2007
Trostchansky A, O´Donnell VB, Goodwin DC, Landino LM; Marnett LJ, Radi R and Rubbo H .Interactions between nitric oxide and peroxynitrite during prostaglandin endoperoxide h synthase-1 catalysis: a free radical mechanism of activation. Free Radical Biology and Medicine. 43: 1029-1038, 2007
Tórtora V, Quijano C, Freeman BA, Radi R and Castro L. Mitochondrial aconitase reaction with nitric oxide, S-nitrosoglutathione and peroxynitrite: Defining the mechanisms and relative contribution of these species to aconitase inactivation. Free Radical Biology and Medicine. 42: 1075-1088, 2007
Piacenza L, Irigoín F, Alvarez MN, Peluffo G, Taylor MC, Kelly JM, Wilkinson S R. and Radi R. Mitochondrial superoxide radicals mediate programmed cell death in Trypanosoma cruzi: cytoprotective action of mitochondrial Fe-superoxide dismutase overexpression. Biochem. J. 403: 323-334, 2007
Ye Y, Quijano C, Robinson KM, Ricart KC, Strayer AL, Sahawneh MA, Shacka JJ, Kirk M, Barnes S, Accavitti-Loper MA, Radi R, Beckman JS, Estevez AG. Prevention of Peroxynitrite-induced Apoptosis of Motor Neurons and PC12 Cells by Tyrosine-containing Peptides. J Biol Chem. 282(9):6324-37, 2007
Demicheli V, Quijano C, Alvarez B and Radi. R. Inactivation and nitration of human SOD by fluxes of nitric oxide and superoxide. Free Radical Biology and Medicine. 42 : 1359–1368, 2007
Szabó C, Ischiropoulos H and Radi R. Peroxynitrite: biochemistry, pathophysiology and development of therapeutics. Nature Reviews Drug Discovery. 6: 662-680, 2007.
Peluffo G. and Radi R Biochemistry of Protein Tyrosine Nitration in Cardiovascular Pathology.
Cardiovascular Research 75: 291-302, 2007
Pehar M.; Vargas MR., Robinson KM, Cassina P, Díaz-Amarilla PJ, Hagen TM, Radi R, Barbeito L, Beckman JS. Mitochondrial superoxide production and Nrf2 activation in p75NTR-induced motor neuron apoptosis. J Neurosci 27, 7777-7785, 2007
Trujillo M, Ferrer-Sueta G, Thomson L, Flohé L and Radi R. Kinetics of peroxiredoxins and their role in the decomposition of peroxynitrite. In: Peroxiredoxin Systems (L Flohé and JR Harris, eds.). Springer, pp. 83-113, 2007
Alvarez MN, Peluffo G, Folkes L, Wardman P, Radi R. Reaction of the carbonate radical with the spin trap 5,5-dimethyl-1-pyrroline-N-oxide in chemical and cellular systems: pulse radiolysis, electron paramagnetic resonance and kinetic-competition studies. Free. Radical Biol. Med. 43, 1523–1533,2007
Trujillo M, Clippe A, Manta B, Ferrer-Sueta G, Smeets A, Declercq JP, Knoops B and Radi R. Pre-steady state kinetic characterization of human peroxiredoxin 5: taking advantage of trp 84 fluorescence increase upon oxidation. Arch. Biochem. Biophys. 467, 95-106,2007
Baker LMS, Baker PRS, Golin-Bisello F, Schopfer FJ, Fink M, Woodcock SR, Branchaud BP, Radi R and Freeman BA. Nitro-fatty acid reaction with glutathione and cysteine:kinetic analysis of thiol alkylation by a michael addition reaction. J Biol Chem. 282, 31085–31093, 2007
Quijano C, Castro L, Peluffo G, Valez V and Radi R. Enhanced mitochondrial superoxide in hyperglycemic endothelial cells: Direct measurements and formation of hydrogen peroxide and peroxynitrite Am. J. Physiol. Heart and Circulatory Physiology. 293(6):H3404-14,2007
Chifflet, S. and Hernandez, J. A. (2007). Use of standard fluorescence microscopy to assess modifications in the plasma membrane potential and in the intracellular concentration of inorganic ions in cultured cells. pp. 229-236. In Modern Research and Educational Topics in Microscopy, vol. 1 (eds. A. Mendez Vilas and J. Diaz Alvarez): Formatex.
Romero N, Peluffo G, Bartesaghi S, Zhang H, Joseph J, Kalyanaraman B, Radi R. Incorporation of the hydrophobic probe N-t-BOC-L-tyrosine tert-butyl ester to red blood cell membranes to study peroxynitrite-dependent reactions. Chem. Res. Toxicol. 20(11):1638-1648, 2007
Turell L, Botti H, Carballal S, Ferrer-Sueta Gerardo, Souza J, Durán R; Freeman B, Radi R and Alvarez B. Reactivity of sulfenic acid in human serum albumin. Biochemistry. 47(1):358-67, 2008
Piacenza L, Peluffo G, Alvarez MN, Nelly JM, Wilkinson SR and Radi R. Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously-derived peroxynitrite. Biochem. J. 410(2):359-68, 2008
Carballal S, Madzelan P, Zinola CF, Graña M, Radi R, Banerjee R, and Alvarez B. Dioxygen Reactivity and Heme Redox Potential of Truncated Human Cystathionine b-Synthase. Biochemistry, 47(10):3194-3201, 2008
González-Pérez S, Quijano C, Romero N, Melø TB, Radi R, Arellano JB. Peroxynitrite inhibits electron transport on the acceptor side of higher plant photosystem II Arch. Biochem. Biophys. 473, 25–33, 2008
Cassina P, Cassina A, Pehar M, Castellanos R, Robinson KM, Beckman JS, Mason RP, Barbeito L and Radi R. Mitochondrial Dysfunction In SOD1G93A-Bearing Astrocytes Promote Motor Neuron Degeneration: Prevention By Mitochondrial-Targeted Antioxidants J. Neurosci. 28(16):4115-22, 2008
Rubbo H and Radi R. Protein and Lipid Nitration: Role in Redox Signaling and Injury. Biochim Biophys Acta. 1780(11):1318-24, 2008
Souza J, Peluffo G and Radi R. Protein tyrosine nitration- functional alteration or just a biomarker? Free. Radical Biol. Med. 45(4):357-66, 2008
Trujillo M, Ferrer-Sueta G and Radi R. Kinetic Studies on Peroxynitrite Reduction by Peroxiredoxins Methods in Enzymology, 441:173-96, 2008
Souza J, Castro L, Cassina A, Batthyany C and Radi R. Nitrocytochrome c: Synthesis,Purification, and Functional Studies. Methods in Enzymology, 441:197-215, 2008
Bartesaghi S, Peluffo G, Zhang H, Joseph J., Kalyanaraman B, Radi R. Tyrosine Nitration, Dimerization, and Hydroxylation by Peroxynitrite in Membranes as Studied by the Hydrophobic Probe N-t-BOC-l-tyrosine tert-Butyl Ester Methods in Enzymology, 441:217-36, 2008
Trujillo M, Ferrer-Sueta G and Radi R. Peroxynitrite detoxification and its biological implications. Antioxidants and Redox Signaling. 10: 1-13, 2008
Irigoín F, Cibils L, Comino M, Wilkinson S, Flohe L, and Radi R. Insights into the redox biology of Trypanosoma cruzi: Trypanothione metabolism and oxidant detoxification. Free Radical Biology and Medicine. 45:733-42, 2008
Stadler K, Bonini MG, Dallas S, Jiang J, Radi R, Mason RP and Kadiiska MB. Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes. Free Radic Biol Med. 45:866-74, 2008
Batinić-Haberle I, Cuzzocrea S, Rebouças JS, Ferrer-Sueta G, Mazzon E, Di Paola R, Radi R, Spasojević I, Benov L and Salvemini D. Pure MnTBAP selectively scavenges peroxynitrite over superoxide: Comparison of pure and commercial MnTBAP samples to MnTE-2-PyP in two different models of oxidative stress injuries, SOD-specific E. coli model and carrageenan-induced pleurisy. Free Radical Biology and Medicine 46(2):192-201, 2009
Abriata LA, Cassina A, Tórtora V, Marín M, Souza JM, Castro L, Vila AJ and Radi R. Nitration of solvent-exposed tyrosine-74 on cytochrome c triggers heme iron-methionine-80 bond disruption: Nuclear magnetic resonance and optical spectroscopy studies. J. Biol. Chem. 284(1):17-26, 2009
Irigoín F, Inada NM, Fernandes MP, Piacenza L, Gadelha FR, Vercesi AE, Radi R. Mitochondrial calcium overload triggers complement-dependent superoxide-mediated programmed cell death in Trypanosoma cruzi. Biochem J. 418(3):595-604, 2009
Disruption of the M80-Fe ligation stimulates the translocation of cytochrome c to the cytoplasm and nucleus in non-apoptotic cells. Godoy LC., Muñoz-Pinedo C, Castro L, Cardaci S, Schonhoff C M, King M, Tórtora V, Marín M, Miao Q, Jiang JF, Kapralov A, Jemmerson R, Silkstone GG, Patel JN, Evans J, Wilson MT, Green DR., Kagan VE., Radi R, Mannick JB. Proceedings of the National Academy of Sciences (USA). 106(8):2653-8, 2009
Ferrer-Sueta G, Radi R. Chemical biology of peroxynitrite: kinetics, diffusion, and radicals. ACS Chem Biol. 4(3):161-77, 2009
Turell L, Carballal S, Botti H, Radi R, Alvarez B.Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment. Braz J Med Biol Res. 42(4):305-11, 2009.
Radi R. Peroxynitrite and reactive nitrogen species: The contribution of ABB in two decades of research [Editorial]. Arch Biochem Biophys. 484: 111-113, 2009
Turell L, Botti H, Carballal S, Radi R, Alvarez B. Sulfenic acid—A key intermediate in albumin thiol oxidation. J. Chromatography B. 877, 3384–3392, 2009
Peluffo G, Calcerrada P, Piacenza L, Pizzano N, and Radi R. Superoxide-mediated inactivation of nitric oxide and peroxynitrite formation by tobacco smoke in vascular endothelium: studies in cultured cells and smokers. Am. J. Physiol. Heart and Circulatory Physiol. 296: H1781–H1792, 2009.
Nin, V., Hernandez, J. A. and Chifflet, S. (2009). Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture. Cell Motil Cytoskeleton 66, 1087-99.
Trostchansky A, Moller M, Bartesaghi S, Botti H, Denicola A, Radi R and Rubbo H.Nitric oxide redox biochemistry in lipid environments. In: Nitric Oxide (Louis Ignarro, Ed). 2nd Edition, Elsevier. In Press, 2009
Trujillo M, Alvarez B, Souza JM, Romero N, Castro L, Thomson L, Radi R. Mechanisms and biological consequences of peroxynitrite-dependent protein oxidation and nitration. In: Nitric Oxide (Louis Ignarro, Ed). 2nd Edition, Elsevier. In Press, 2009
Souza JM, Bartesaghi S, Peluffo G and Radi R. Nitrotyrosine: Quantitative analysis, mapping in proteins and biological significance. In: Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications (Aldini G. , Yeum KJ, Niki E, Russell RM, Eds). Wiley-Blackwell. In Press, 2009
Piacenza L, Zago MP, Peluffo G, Alvarez MN, Basombrio MA and Radi R. Enzymes of the antioxidant network as novel determiners of Trypanosoma cruzi virulence. Int. J. Parasitol. 39: 1455–1464, 2009
Piacenza L, Alvarez MN, Peluffo G, and Radi R. Fighting the oxidative assault: the Trypanosoma cruzi journey to infection. Curr. Opinion. Microbiol. 12: 415-421, 2009
Kawai C, Pessoto F, Rodrigues T, Katia M, Tórtora V, Castro L, Milícchio V, Tersariol I, Di Mascio P, Radi R, Carmona-Ribeiro A and Nantes I. pH-Sensitive Binding of Cytochrome c to the Inner Mitochondrial Membrane. Implications for the Participation of the Protein in Cell Respiration and Apoptosis. Biochemistry. 48:, 8335–8342, 2009
Hugo M, Turell L, Manta B, Botti H, Monteiro G, Netto LES, Alvarez B, Radi R and Trujillo M. Thiol and Sulfenic Acid Oxidation of AhpE, the One-Cysteine Peroxiredoxin from Mycobacterium tuberculosis: Kinetics, Acidity Constants, and Conformational Dynamics. Biochemistry. 48: 9416–9426, 2009
Redondo-Horcajo M, Romero N, Martínez-Acedo P, Martínez-Ruiz A, Quijano C, Lourenço CF, Movilla N, Enríquez JA, Rodríguez-Pascual F, Rial E, Radi R, Vázquez J, Lamas S.Cyclosporine A-Induced Nitration of Tyrosine 34 MnSOD in Endothelial Cells: Role of Mitochondrial Superoxide. Cardiovascular Research. In Press, 2010
Hernandez-Saavedra D, Quijano C, Demicheli V, Souza JM, Radi R, McCord JM. Thiol-sensitive mutant forms of human SOD2, L60F and I58T: The role of Cys140. Free Radical Biology and Medicine, In Press, 2010
Bartesaghi S, Wenzel J, Trujillo M, Lopez M, Joseph J, Kalyanaraman B, Radi R. Lipid Peroxyl Radicals Mediate Tyrosine Dimerization and Nitration in Membranes. Chem. Res. Toxicol. In Press, 2010
